By Harry ed. Sobotka
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Extra info for Advances in Clinical Chemistry, Vol. 4
The method of Timmell et al. 4% o-aminodiphenyl in glacial acetic acid, can be used to measure the lactose concentration in a solution. The amount of glucose present in the sample can be determined by the glucose oxidase method and its contribution to the final color developed deducted. The reaction mixture must be heated to 100°C for 90 minutes for complete color development with lactose. Fructose does not react with this reagent but aldoses do. 6. Sucrose Sucrose is a nonreducing sugar. It can be estimated by acid hydrolysis followed by determination of glucose and/or fructose.
Because of its flexibility and the fact that the color can be developed in a 37°C water bath, we prefer the method of Pogell. This method has been modified to yield a stable colored product (D2). 04% skatole in 95% ethanol and then 8 ml cold 30% HCl are added. The contents are mixed and incubated at 38°C. The reaction is stopped by removing the tubes to an ice bath and immediately adding 10 ml chloroform to extract the color. The time of incubation can be varied to accommodate the con- 38 JAMES B.
Gr. 75). 4 mM KH2P04. Sodium Solutions, 200 mM NaC1; 14 mM NaC1. Magnesium Standards for Plasma, Urine, and Feces. Solutions are prepared by adding 10 ml mixed salt solution A, 10 ml calcium solution, and an appropriate volume of magnesium solution I to 700 ml water in a liter flask. Perchloric acid (50 ml) is now added, and the volume is made up to 1 liter. 40 mM with respect to magnesium concentration. Calcium Standards for Plasma, Urine, and Feces, Ten milliliters of mixed salt solution A, 10 ml magnesium solution 11, and an appropriate volume of calcium solution are added to 700 ml water in a liter flask.